Senegal played without their injured star man Sadio Mane and fell to the Dutch.
Senegal lost their first World Cup match at Qatar 2022 after two late goals by Cody Gakpo and Davy Klaassen gave the Netherlands a 2-0 victory.
The African champions played on Monday without their talisman, Sadio Mane.
AFP reported that the first goal came just six minutes remaining when PSV Eindhoven forward Gakpo got up in front of Senegal goalkeeper Edouard Mendy to head in a floated Frenkie de Jong cross from the left.
Substitute Klaassen then secured the victory deep in injury time, following up to score after Mendy had saved from Depay, who had also come off the bench.
The result leaves the Netherlands level at the top of the group with Ecuador, who beat hosts Qatar 2-0 in the tournament’s opening match on Sunday.
Louis van Gaal’s side play Ecuador on Friday, while Senegal now face a must-win contest against Qatar.