Ronaldo inspired Juventus as they hammered Barcelona 3-0 at home in Spain to go top of their Champions League group.
Ronaldo and Messi
Juventus on Tuesday, December 9, 2020, outclassed their rival, Barcelona in the Champions League to win by three goals.
Cristiano Ronaldo scored two penalties as his team went top of Group G
According to BBC, in his club career, Cristiano Ronaldo has scored more away goals against Barcelona than he has against any other opponent (14)
Ronaldo sent Marc-Andre ter Stegen the wrong way after being fouled by Uruguay defender Ronald Araujo.
Juve doubled their lead through Weston McKennie's bicycle kick before Ronaldo added his second from the spot after Clement Lenglet handled.
"We weren't aggressive," said Barca boss Ronald Koeman.
"It looked as if we'd come out looking not to lose, as opposed to trying to control the game. We weren't good, not even positionally.
"We looked scared."
It is Barcelona's first defeat in a group game since 2016.
They started the day with a three-point cushion over Juve but finish second and will be unseeded in Monday's last-16 draw.
Barcelona forward Lionel Messi and Ronaldo, regarded as the two greatest players of their generation, were facing one another for the first time since May 2018.
While Ronaldo scored two, Messi was frustrated by 42-year-old Juve keeper Gianluigi Buffon, who made a string of fine saves.
Juventus finish top of Group G because of their superior head-to-head record over Barcelona
This was the 36th meeting between great rivals Messi and Ronaldo on the international, European and domestic stage.
They have won the Champions League nine times between them, scoring a combined 252 goals in the competition.
But this was Ronaldo's night, the Portuguese scoring for the first time in the Champions League against Barcelona with Messi on the pitch.
Twelve years on from their first meeting, Ronaldo, the all-time leading scorer in Champions League history, got the better of his arch rival.
Lionel Messi had all seven of Barcelona's shots on target in this match
Earlier in the 2020-21 season, Messi became the first player to score in 16 successive Champions League campaigns.
Messi had seven shots on target but could find no way past Buffon, while Antoine Griezmann headed against the bar.
Griezmann said the defeat was not Koeman's fault.
"Who else's fault could it be other than the players? We're the ones who go out there and play," he said.
"We were missing everything - desire, attitude, a willingness to run. Everything was bad."
At the age of 35, Ronaldo might not get too many more chances to cross swords with Messi.
He still trails Messi 16-11 in terms of victories between the two but this was a night to savour for Juventus' former Real Madrid player.